Resource Number: 14635
Title: Validation of PCR-Based Assays and Laboratory Accreditation for Environmental Detection of Aquatic Invasive Species
Publisher: US Department of the Interior
Description: This white paper provides:
a) Background information on the use, accuracy and reliability of pcr-based assays such as environmentally sampled dna (edna) for early detection of aquatic invasive species (ais) and;
b) Recommendations for establishing a system for validating assays and accrediting laboratories that report on the presence or absence of ais.
This white paper was developed by the members of isac and discusses the need for developing validation requirements for Polymerase Chain Reaction (pcr) and other dna-based molecular assays that are increasingly being used to detect ais. It does not provide a simplified checklist for evaluation of their ability to detect ais. Rather, it is intended to demonstrate the need for a required and regulated framework to validate these molecular assays. A regulated framework for validation would greatly increase confidence in the utility of dna-based assays and better enable decision-makers and managers regarding
ais detection, prevention, monitoring and control.
Last Updated: June 24, 2019
Contributors:
Domain(s)/Area(S):
- Natural Resources
- Pathways
Locations: